“Jimmy Kimmel Live!” guest host Rosie O’Donnell opened her third night of hosting the show with a message to President Donald Trump on a topic that has been irking MAGA figures lately.

“A special hello to those of you watching from home – and from the White House. But I do have some bad news for President Petty Roosevelt,” O’Donnell said.

“That’s all I’m going to say about you tonight, sweetheart. You’ve got to realize it’s time to hang up your wig and let Natalie tuck you in. Time to go nighty-night,” she continued, referencing White House aide Natalie Harp.

Trump and O’Donnell have been feuding for the last two decades, with the president once referring to her as a “fat pig,” a “slob” and an “animal.”

O’Donnell joined in on this week’s trend of discussing Harp’s relationship with the president, after Sen. Jon Ossoff’s comments over the weekend put her in the national spotlight. Since then, many Trump allies have been lashing out at any suggestion that Harp, dubbed the “human printer” by White House colleagues, has an inappropriate relationship with him.

“Nobody gives a shit what this lightweight loser says,” White House spokesperson Davis Ingle wrote on X about Ossoff.

“Jon Jackoff has to be the biggest cuck loser in politics. Instead of denigrating hard working people serving their country, Jon should look deep into his soul and ask himself why he’s a miserable person who hates this country. It’s because he’s a radical, extremist Dumocrat,” White House communications director Steven Cheung wrote on X.

Much about Harp has come to light over the past few days, including reports from CNN that she got into a “screaming match” with the Secret Service over not getting a seat in Trump’s SUV, prompting her to jump in the trunk to stay close to him. Harp also reportedly worked at the White House for more than a year without proper security clearance, according to an MS NOW report.

Watch O’Donnell’s full opening monologue from Wednesday night’s show here:

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